Species: Common Carp (Cyprinus carpio)

Country of 1st Author: China
Year: 2016

Institute	Soochow University Suzhou 215123, Jiangsu CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	TALEN pairs each for runx2, sp7a, mstnba and spp1a were microinjected into the one-cell common carp embryos at a concentration of 250?ng/ul per arm, respectively
Remarks	Cas9 mRNAs plus the six individual gRNAs for corresponding genes sp7a, sp7b, mstnba, runx2, opga and bmp2ab were co-microinjected into one-cell common carp embryos, respectively
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
sp7 sp7 transcription factor	a zinc-finger-containing transcription factor expressed in osteoblasts, which can activate pre-osteoblast cells to differentiate into mature osteoblast cells and osteocytes	Other	TALEN
runx2 runt-related transcription factor 2	an essential transcription factor to regulate osteoblast differentiation and bone developmen	Other	TALEN
spp1 secreted phosphoprotein 1	a late osteoblast specific marker involved in bone mineralization	Other	TALEN
MSTN Myostatin	Double muscle	Yield	TALEN
sp7 sp7 transcription factor	a zinc-finger-containing transcription factor expressed in osteoblasts, which can activate pre-osteoblast cells to differentiate into mature osteoblast cells and osteocytes	Other	CRISPR Cas9 (spCas9)	sp7a and sp7b
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	mstnba
runx2 runt-related transcription factor 2	an essential transcription factor to regulate osteoblast differentiation and bone developmen	Other	CRISPR Cas9 (spCas9)
opg osteoprotegerin	a bone-protecting molecule participated in impairing the osteoclast formation	Other	CRISPR Cas9 (spCas9)
bmp2 Bone morphogenetic protein 2		Other	CRISPR Cas9 (spCas9)	bmp2ab

References, Documents, and Links

• Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp