Species: Atlantic Salmon (Salmo salar)

Country of 1st Author: Norway
Year: 2020

Institute	Institute of Marine Research Nordnes, NO-5817 Bergen NO
Editing Method	Embryo edit
SDN Type	SDN-2
Delivery	Fertilization and microinjections were carried out using 50?ng/µl gRNA and 150?ng/µl cas9 mRNA . The ODNs (S, AS or ds) were added to the injection mix with a final concentration of 1.5 or 0.15?µM, and the plasmid with a final concentration of 2.5 or 10?ng/µl (corresponding to 0.001 and 0.004?µM, respectively).
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
slc45a2 solute carrier family 45 member 2		Colour	CRISPR Cas9 (spCas9)	Targeting the pigmentation gene slc45a2, we have performed KI of a FLAG element in F0 salmon using CRISPR/Cas9 and symmetrical DNA repair templates (Fig. 1). Wierson et al.39 have shown that short homology arms from 24–48?bp is enough to direct precise and efficient knock-in in zebrafish. Based on this we have tested 24, 48 and 84?bp homology arms in this study.

References, Documents, and Links

• Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon