Species: Pig (Sus scrofa domesticus)

Country of 1st Author: South Korea
Year: 2019

Breed Strain	Yucatan miniature
Institute	Optipharm Inc Cheongju 28158 KR
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	Approximately 1 × 106 fetal fibroblasts weretransfected with 1.7 µg pX330-501 vector and 3.3 µg donor vector. After 48 h, the cells were transferred to ten 10-cm plates with selective medium containing G418 (600 µg/mL, promega). After 10 days, the resistant colonies were selected and expanded, and a smallsample of every colony was subjected togenotyping.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
GGTA1 α-1,3-galactosyltransferase	Hypoallergenic meat	Hypoallergenic	TALEN	Exon 9 of porcine GGTA1 gene (GenBank accession no. AH010595.2) was chosen for TALEN construct with 5'-GAG GAG TTC TTA A-3' for left TALE, 5'-GGC CAC AAA GTC ATC TTT TAC A-3' for right TALE

References, Documents, and Links

• No excessive mutations in transcription activator-like effector nuclease-mediated a-1,3-galactosyltransferase knockout Yucatan miniature pigs