Species: Atlantic Salmon (Salmo salar)

Country of 1st Author: Norway
Year: 2020

Institute	Institute of Marine Research Nordnes, NO-5817 Bergen NO
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Microinjection
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
dnd dead end	Germ cell deficient	Reproduction	CRISPR Cas9 (spCas9)	Embryos were injected with a mixture of 50 ng/ml dnd gRNA, 50 ng/ml slc45a2 gRNA, 150 ng/ml Cas9 mRNA and 100 ng/ml dnd, or one gRNA targeting dnd and Cas9 mRNA together with dnd mRNA

References, Documents, and Links

• Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon