Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2022

Institute	Chinese Academy of Sciences, Beijing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	direct zygote microinjection of the CBE system can generate pigs harboring multiple point mutations.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	DNA base editor
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	DNA base editor
IGF2 Insulin-like growth factor 2	Muscle development	Yield	DNA base editor

References, Documents, and Links

• One-step base editing in multiple genes by direct embryo injection for pig trait improvement