Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2017

Breed Strain	Chinese Indigenous Erhualian
Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	PFFs at low passages were electroporated with 20 µg Cas9/gRNA-1 and 20 µg Cas9/gRNA-2 using BTX ECM 2001 square-wave electroporator
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs