Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2021

Institute	Jilin University, Changchun Jilin Province CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
FST Follistatin	Increased skeletal muscle mass	Yield	CRISPR Cas9 (spCas9)	site-specific integration of FSI-I-I, including the signal peptide, N terminus, and three FSI domains, into the last codon of the porcine MSTN gene using a homology-mediated end joining (HMEJ)-based strategy mediated by CRISPR-Cas9

References, Documents, and Links

• HMEJ-mediated site-specific integration of a myostatin inhibitor increases skeletal muscle mass in porcine