Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2018

Institute	Jilin University, Changchun Jilin Province CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Delivery	Approximately 30 mg ofpX330-sgRNA91 plasmid and 30 mgofdonor puc57-fat-1-KI plasmid were cotransfected into 3·106 PFFs using the BTX-ECM 2001 Electroporation system.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
fat-1 Omega-3 fatty acid desaturase fat-1		Quality	CRISPR Cas9 (spCas9)	the fat-1 gene was inserted into porcine Rosa 26 (pRosa26) locus via Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) system.

References, Documents, and Links

• Site-Specific Fat-1 Knock-In Enables Significant Decrease of n-6PUFAs/n-3PUFAs Ratio in Pigs