Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2021

Institute	Jilin University, Changchun Jilin Province CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Delivery	electroporation of plasmids into PFFs
Remarks	In this study, pigs were genetically modified with multiple genes that were precisely inserted into the pRosa26 locus by using the clustered regularly spaced short palindrome repeat sequence (CRISPR)/CRISPR-related 9 (Cas9) system and somatic cell nuclear transfer technology (SCNT) in combination
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
fat-1 Omega-3 fatty acid desaturase fat-1		Quality	CRISPR Cas9 (spCas9)
IGF1 Insulin Like Growth Factor 1	manages the effects of growth hormone (GH) in the body	Quality	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus