Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2020

Institute	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	we report generation of double-gene-knockout (DKO) pigs harboring edited knockout alleles for known receptor proteins CD163 and pAPN and show that DKO pigs are completely resistant to genotype 2 PRRSV and TGEV
Remarks	For CD163, the SRCR5 domain-binding site for PRRSV in exon 7 a single guide was selected. For pAPN a sguide target site in exon 2
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)
pAPN	Resistance against enteric coronaviruses	Biotic Stress	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance