Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2018

Institute	South China Agricultural University, Guangzhou, China; Wens Foodstuff Group Co., Ltd. Yunfu CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	we described the generation of a CD163 knockout (KO) pig in which the CD163 protein was ablated by using CRISPR/Cas9 gene targeting and somatic cell nuclear transfer (SCNT) technologie
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	Single gRNA targeting regions within the exon 7

References, Documents, and Links

• CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus