Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2016

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Approximately 20 ng/µ L of Cas9 mRNA and 5 ng/µ L of each sgRNA from the MSTN, ASIP, and BCO2 genes were pooled and microinjected into 20 sheep embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)
ASIP agouti signaling protein	Coat color change	Colour	CRISPR Cas9 (spCas9)
BCO2 beta-carotene oxygenase 2	This gene encodes an enzyme which oxidizes carotenoids such as beta-carotene during the biosynthesis of vitamin A.	Other	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep