Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2022

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Microinjection of 100 ng/ µL Cas9 mRNA and 200 ng/µL sgRNAs
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	Ten sgRNAs targeting the MSTN gene were selected and validated in vitro using sheep fibroblast cells

References, Documents, and Links

• Optimized Cas9:sgRNA delivery efficiently generates biallelic MSTN knockout sheep without affecting meat quality