Chinese Scientists Develop Improved CRISPR SystemJuly 18, 2018
The CRISPR-Cas9 system has been widely used for inducing targeted mutations in a variety of species. In Arabidopsis, CRISPR relies on the edited cells where the Cas9 protein does its activity to obtain heritable and stable mutated lines. Fudan University researchers designed an improved CRISPR-Cas9 system, named MSC (meiocyte-specific CRISPR-Cas9) system. In this system, the Cas9 expression is driven by an experimentally approved meiocyte-specific promoter.
To test the system, the team, led by Penghui Xu, targeted two endogenous genes, the vegetative gene AtDET2, and reproductive gene AtDMC1. In the T1 generation, heterozygous plants for the targeted genes were obtained in high efficiency. Meanwhile, homozygous plants were abundant in the T2 generation.
The team also made a suicide-MSC system, which can edit the targeted endogenous gene and the exogenous Cas9 gene simultaneously, maintaining the stable mutated alleles for functional studies.
These two new systems provide new approaches to generate mutations for gene functional studies.
For more information, read the article in Frontiers in Plant Science.
See more articles:
News from Around the World
- Experts Tackle Importance of GM Crops Commercialization in Beijing
- African Scientists Challenged to Invest in Developing Communication Skills
- International Research Team Discovers Genetic Mechanism that Allows Rice to Survive Flooding
- Farm Babe Debunks Myths about GMOs
- Japanese Researchers Assemble First Accurate Buckwheat Genome
- Cornell Alliance for Science Calls for Comments for Golden Rice in PH
- Vietnamese Farmers Share Experiences in GM Maize Planting
- Socio-economic Aspect of GM Crops Highlighted in Symposium
- Australian OGTR Issues License for GM Wheat Field Trials
- EFSA Publishes Scientific Opinion on Four-Event Stack Maize Bt11 × MIR162 × 1507 × GA21
- Sugarcane Genome Has Finally Been Sequenced
- ZmNBS25 from Maize Functions as a Disease Resistance Gene Across Species
- Upregulation of Lipid Biosynthesis Increases the Oil Content in Sorghum
Plant Breeding Innovations
- Researchers Find Soybean Genes for Alkaline Stress Resistance
- FaTM6 Controls Flower Development in Strawberry
- OsIPMS1 Gene Involved in Seed Vigor and Energy Metabolism in Rice
- Chinese Scientists Develop Improved CRISPR System
From the BICs
- "Pink Bollworm Management Strategy" Book Now Available in Telugu
- CRISPR Can Speed up Nature and Change How We Grow Food
Subscribe to CBU: