Crop Biotech Update

FaTM6 Controls Flower Development in Strawberry

July 18, 2018

The B-class of MADS-box transcription factors has been studied in many plant species, but remain uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of this class, is known to control the petals and stamens in Arabidopsis thaliana. Carmen Martín-Pizarro, together with her colleagues from the Universidad de Malaga in Spain, aims to learn AP3's role in the cultivated strawberry (Fragaria × ananassa) by studying an AP3 member, FaTM6.

Using CRISPR-Cas9 system, the team aimed to knockout the FaTM6 to characterize the function of FaTM6 in flower development. An analysis of the target sites showed a high efficiency genome editing already in the T0 generation. Phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in petal and especially in anther development in strawberry.

These results validate that FaTM6 plays a role in flower development in strawberry. This study also confirms that CRISPR-Cas9 can be applied in an octoploid species such as the cultivated strawberry.

For more information, read the article in the University de Malaga website.