FaTM6 Controls Flower Development in Strawberry

The B-class of MADS-box transcription factors has been studied in many plant species, but remain uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of this class, is known to control the petals and stamens in Arabidopsis thaliana. Carmen Martín-Pizarro, together with her colleagues from the Universidad de Malaga in Spain, aims to learn AP3's role in the cultivated strawberry (Fragaria × ananassa) by studying an AP3 member, FaTM6.

Using CRISPR-Cas9 system, the team aimed to knockout the FaTM6 to characterize the function of FaTM6 in flower development. An analysis of the target sites showed a high efficiency genome editing already in the T0 generation. Phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in petal and especially in anther development in strawberry.

These results validate that FaTM6 plays a role in flower development in strawberry. This study also confirms that CRISPR-Cas9 can be applied in an octoploid species such as the cultivated strawberry.

For more information, read the article in the University de Malaga website.


 

This article is part of the Crop Biotech Update, a weekly summary of world developments in agri-biotech for developing countries, produced by the Global Knowledge Center on Crop Biotechnology, International Service for the Aquisition of Agri-Biotech Applications SEAsiaCenter (ISAAA)

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