Papain Inhibitor Contributes to Phytophthora palmivora Infection in PapayaFebruary 7, 2018
Papaya trees are rich in papain, a cysteine protease that has been shown to mediate plant defense against pathogens and insects. However, the pathogen Phytophthora palmivora infects all parts of papaya plants, suggesting that it has evolved cysteine protease inhibitors to counter papain and enable infection.
Out of several cystatin-like cysteine protease inhibitors from P. palmivora, PpalEPIC8 appeared to be unique to P. palmivora and was highly induced during infection of papaya. Analysis revealed that PpalEPIC8 strongly inhibited papain enzyme activity, suggesting that it is a cysteine protease inhibitor. Phytophthora palmivora mutants with knocked-out PpalEPIC8 were the generated using CRISPR-Cas9-mediated gene editing.
The mutant strains exhibited increased papain sensitivity in vitro and had reduced pathogenicity during infection of papaya fruits compared to the wild-type strain. This strongly suggests that PpalEPIC8 indeed plays a role in P. palmivora virulence by inhibiting papain.
This study provides evidence of pathogens secreting cystatins to invade plants. It also established a gene-editing system for P. palmivora using CRISPR-Cas9.
For more on this study, read the article in Molecular Plant-Microbe Interactions.
See more articles:
News from Around the World
- High Adoption of Biotech Crops Recorded in 2016
- Rwandan Researchers Discuss GM Crop Adoption
- University of Toronto Study Says Root Microbiome Key to Drought Resistant Plants
- Graduate Students Request to Deregulate Process of GM Crop Development
- "Tomato Expression Atlas" Provides Deeper Understanding of the Crop
- USDA and FDA Commit to Work Together to Ensure Food Safety
- Bangladeshi Professionals Show Positive Attitude on Biotech Products
- Research Group Finds Root Growth and Cell Replenishment Mechanisms
- Plague-Resistant Tomatoes Developed through Genetic Engineering
- Gene from Commerson's Wild Potato Confers Significant Freezing and Drought Tolerance in Arabidopsis
- VviAGL11 Gene Regulates Seed Morphogenesis in Grapevine
Plant Breeding Innovations
- Scientists Perform Simultaneous Editing of Duplicated Loci in Soybean
- Papain Inhibitor Contributes to Phytophthora palmivora Infection in Papaya
- Researchers Generate Arabidopsis GGAT1 Mutants Using CRISPR
Beyond Crop Biotech
- Colorado Potato Beetle Genome Gives Insights into the Pest's Adaptability
- Researchers Knockout Multiple Genes in Rabbit via CRISPR-Cas9
- IMF Video: GMOs Can Help World Hunger
- ISAAA in 2017: Accomplishment Report
Subscribe to CBU: