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Crop Biotech Update

Scientists Perform Simultaneous Editing of Duplicated Loci in Soybean

February 7, 2018

The CRISPR-Cas9 system is a powerful tool for site-directed mutagenesis in crops. The team of Yuhei Kanazashi from Hokkaido University in Japan were successful in performing a simultaneous site-directed mutagenesis in two homoeologous loci in soybean (Glycine max), GmPPD1 and GmPPD2. These two genes are orthologs of Arabidopsis thaliana PEAPOD (PPD) gene.

Most of the T1 plants generated had mutations in at least one of the targeted loci. Analysis of T1 generation indicates that the mutations induced in the T0 plant can be inherited by the T1 generation. The team also induced new mutations in T1 plants, which were then also detected in the T2 generation. This indicates that continuous induction of mutations during T1 plant development could increase the occurrence of mutations in germ cells.

Mutations in both GmPPD loci were also confirmed in at least 33% of the T2 seeds examined. The mutants with mutations in both GmPPD1 and GmPPD2 exhibited dome-shaped trifoliate leaves, extremely twisted pods, and produced few seeds.

These results suggest that continuous induction of mutations in the succeeding generations of genome-edited plants can increase the efficiency of mutagenesis in duplicated loci in soybean.

For more information, read the article in Plant Cell Reports.