Researchers Generate Arabidopsis GGAT1 Mutants Using CRISPRFebruary 7, 2018
Glutamate:glyoxylate aminotransferase 1 (GGAT1) is a key enzyme in plants' photorespiration pathway. However, the mechanism of its regulation is unknown. With the generation of mutated ggat1 from the Col-0 genetic background, mutated ggat1 from the Ler genetic background should be useful in the study of GGAT1. However, the latter are not currently available.
The team of Yaping Liang from the South-China Agricultural University used CRISPR-Cas9 to generate ggat1 mutants from the Ler genetic background. The team designed two single-guide RNAs (sgRNAs) that target GGAT1. These were then inserted into flowering Arabidopsis (Ler) plants via Agrobacterium tumefaciens-mediated transformation. Thirteen GGAT1-edited T1 lines were generated from the transformed plants. From these T1 plants, two homozygous T2 mutants were generated.
The mutations were found to be stable through generations. In addition, the genetic segregation of the mutations followed the Mendelian segregation, and no off-target mutations were detected. The two independent ggat1 mutants had similar photorespiration phenotypes and downregulated GGAT enzyme activity.
CRISPR-Cas9 was successful in generating genetically stable Arabidopsis ggat1 Ler mutants. These will be useful in further research studies on GGAT1 enzyme.
For more information on this study, read the article in Transgenic Research.
See more articles:
News from Around the World
- High Adoption of Biotech Crops Recorded in 2016
- Rwandan Researchers Discuss GM Crop Adoption
- University of Toronto Study Says Root Microbiome Key to Drought Resistant Plants
- Graduate Students Request to Deregulate Process of GM Crop Development
- "Tomato Expression Atlas" Provides Deeper Understanding of the Crop
- USDA and FDA Commit to Work Together to Ensure Food Safety
- Bangladeshi Professionals Show Positive Attitude on Biotech Products
- Research Group Finds Root Growth and Cell Replenishment Mechanisms
- Plague-Resistant Tomatoes Developed through Genetic Engineering
- Gene from Commerson's Wild Potato Confers Significant Freezing and Drought Tolerance in Arabidopsis
- VviAGL11 Gene Regulates Seed Morphogenesis in Grapevine
Plant Breeding Innovations
- Scientists Perform Simultaneous Editing of Duplicated Loci in Soybean
- Papain Inhibitor Contributes to Phytophthora palmivora Infection in Papaya
- Researchers Generate Arabidopsis GGAT1 Mutants Using CRISPR
Beyond Crop Biotech
- Colorado Potato Beetle Genome Gives Insights into the Pest's Adaptability
- Researchers Knockout Multiple Genes in Rabbit via CRISPR-Cas9
- IMF Video: GMOs Can Help World Hunger
- ISAAA in 2017: Accomplishment Report
Subscribe to CBU: