Tiny Genome Sequencer Device Completes E. coli Genome
June 17, 2015| |
A team of researchers from Canada and United Kingdom led by Dr. Jared Simpson conducted a study to examine the capability of Oxford Nanopore's MinIONTM as a sequencer device.
The nanopore device is a tiny genome sequencer that has the ability to generate vast amounts of data and read long stretches of genome to allow reconstruction. However, the device produces inaccurate single reads. To overcome this problem, the researchers devised three stages with this device. First, the overlaps are detected between reads and then corrected by multiple-alignment process. Second, the corrected reads are clustered using the Celera assembler. Lastly, assembly is refined using a probabilistic model of the electric signals caused by DNA moving through the nanopore.
Using this method, the researchers were able to sequence and assemble a new full genome of Escherichia coli. This finding provides evidence of the potential use of this technology to sequence genomes in more complex organisms.
Read the details of their study at Ontario Institute for Cancer Research's website.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Report Forecasts Global Seed Market Trends to 2020
- Biotech Expert Calls for Stronger Links for Regulation of GM Products
- Next Harvest II Report Launched in Kenya
- Researchers Discover Microbe Mobilizing 'Iron Shield' Blocking Arsenic Uptake in Rice
- A New Approach in Managing GMO Spread
- A Grass Could Replace Arabidopsis as Model Plant
- Iran to Produce Bt Cotton
- APEC Member Economies Acknowledge Importance of SciCom in Decision-Making Process
- Scientists Close the Gap on Yellow Rust
-
Research Highlights
- Rice Gene OsGRAS23 Confers Drought Stress Response
- Sl3-MMP from Tomato Regulates Defense Responses against Pathogens
-
Beyond Crop Biotech
- Tiny Genome Sequencer Device Completes E. coli Genome
-
Announcements
- Breeding Science Symposium in Korea
- RNAi Product Safety Assessment Workshop
-
Resources
- Quotable Quotes: Why Biotech?
- Award-Winning Book "Seeds for Change: The Lives and Work of Suri and Edda Sehgal"
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet