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Crop Biotech Update

OsPLDβ1 Disruption Improves Salt Stress Tolerance in Rice

August 26, 2026

Researchers from the International Centre for Genetic Engineering and Biotechnology in New Delhi, India, have uncovered the role of rice gene OsPLDβ1 under salt stress. Their findings are published in Frontiers in Genome Editing.

Phospholipase D (PLD) enzymes play a vital role in plant stress signaling. However, because the function of OsPLDβ1 remained unclear, the research team used CRISPR-Cas9 gene editing to investigate it.

Knockout mutants showed greater tolerance to saline conditions than wild-type plants. This improvement was linked to a greater capacity for scavenging reactive oxygen species, resulting in better management of oxidative stress.

The mutant lines also displayed increased expression of genes involved in ion transport and homeostasis, while maintaining photosynthetic efficiency and agronomic performance.

These findings demonstrate that OsPLDβ1 negatively regulates salt stress tolerance in rice and offer valuable insights for developing salinity-resistant crop varieties.

Read more findings in Frontiers in Genome Editing.


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