Therapeutic Antibody Purification from GM Plants Using Protein A-OB
March 16, 2012| |
Purification of therapeutic monoclonal antibodies from genetically modified plants is considered as a low-cost technique with high scalability and ensured product safety. However, it is a challenge to purify antibodies in plants compared with animals due to the need for large quantities of biomass required in the process.
Scientist Michael McLean and team from the University of Guelph in Canada developed and used Protein A oilbodies (OB) technology to purify trastuzumab, an anti-HER2 (human epidermal growth factor receptor 2) therapeutic antibody from Nicotiana benthamiana. They isolated Protein A-OB from transgenic safflower seeds then used to capture human IgG (hIgG) spiked into buffer, hIgG spiked into the wild-type plant extract, and trastuzumab from genetically engineered N. benthamiana plants. With these results, it is verified that Protein A-OB is a promising tool for the purification of antibodies from plants.
Read the abstract at http://www.springerlink.com/content/mu7k002428255jr4/.
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