Scientists from the Dhirubhai Ambani Life Sciences Center (India) investigated the conditions for efficient regeneration of the Jatropha curcas plant (a biodiesel energy crop) from immature embryos. Their findings are published in the journal, Plant Biotechnology Reports (URL above). The size of embryo was found to be critical for the establishment of callus. A good response of morphogenic callus induction (85.7%) and subsequent plant regeneration (70%) with the maximum number of plantlets (4.7/explant) on supplemented Murashige and Skoog's (MS) medium, was obtained when embryos of 1.1cm to 1.5 cm sizes (obtained from the fruits 6 weeks after pollination) were used. A higher frequency of callus induction (100%) and plant regeneration (90%) with the maximum number of plantlets, were obtained when the medium was supplemented with growth adjuvants, such as casein hydrolyzate, L-glutamine and copper sulphate. The rooted plants after acclimatization were reportedly transferred successfully to the field in different agro-climatic zones in India. The protocol was also found successful on five elite lines of J. curcas..