Researchers in Japan Develop Genome Editing Method that Partially Inihibits Gene Function

A research team led by Associate Professor Takashi Ishida from Kumamoto University in Japan reveals a new genome editing breakthrough that enables partial inhibition of gene function using CRISPR-Cas9 technology. The findings of their study are published in the Journal of Plant Research.

Researchers often face challenges studying essential genes, as completely disabling them can be fatal to organisms. To address this, the research team developed a method that introduces hypomorphic mutations that reduce and do not eliminate gene function. Using Arabidopsis thaliana, they applied this approach to HPY2, a gene essential for cell division and plant growth, which resulted in delayed growth.

This technique, which fine-tunes gene activity instead of disabling it, could help enhance crop traits like stress resistance, making plants more resilient to climate change without compromising growth and yield. “It also opens up new possibilities for precision breeding in agriculture, where controlled gene modification is key to improving traits without unintended consequences,” Associate Professor Ishida said.

For more information, read the abstract from the Journal of Plant Research or the article from Kumamoto University.