The SEED/Harvest Method Allows Efficient DNA Modification
August 7, 2024| |
Researchers from Biozentrum at the University of Basel produced the SEED/Harvest method, which is a more precise and efficient version of CRISPR-Cas9.
CRISPR-Cas has various applications in animal research. However, it is difficult to conduct knock-in generation in multicellular organisms, which may be due to the intricacy of insertion screening.
Scientists combined CRISPR-Cas9 and Single-Strand Annealing (SSA) repair pathway to develop the SEED/Harvest method. This novel technique allows changes in the whole genome of the organism without causing unwanted scars.
The SEED/Harvest method works by introducing a marker gene at the chosen DNA location within a protein-coding region. The marker is then removed, and the breakpoints are repaired by the SSA. The blend of the two techniques enables the marking of any protein in the genome without damage, so the function of proteins can be analyzed. The method has been successfully tested in fruit flies (Drosophila melanogaster) and may be used for further research.
Read the press release of Biozentrum for more information.
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