Scientists Found a Way to Level Up Nonviral Gene EditingMay 17, 2023
Scientists from Chris Richardson's lab at UC Santa Barbara reported a new method that boosts the efficiency of CRISPR-Cas9 editing without needing a viral material to transport the template that edits the target gene sequence. Their study is published in Nature Biotechnology.
Viruses are often used to deliver the repair template DNA to the cell's nucleus. While viruses are effective, viral overflows are pricey, difficult to scale, and could be toxic to cells. “We've found a chemical modification that improves nonviral gene editing and also discovered an intriguing new type of DNA repair,” Richardson said.
Nonviral templates could be cheaper and more scalable, but there are still challenges that need to be addressed, such as efficiency and toxicity barriers. They also found that introducing interstrand crosslinks into the workflow increased homology-directed repair by about threefold. This breakthrough enables researchers to create disease models and test hypotheses about how disease works, and bring about better clinical and therapeutic strategies.
Read more from UC Santa Barbara.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- Genome Editing in Potato using TALENs Delivered via Agroinfiltration
- TALENs-mediated Editing in Potato via Agroinfiltration
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Bayer Submits Application for Second Renewal for Import and Processing of GM Maize MON810 in The Netherlands
- CIMMYT's Alison Bentley Announced as 2023 Borlaug CAST Communication Awardee
- ISAAA Inc. to Hold ASCA6 on September 11-15 in Indonesia
- Queensland University of Technology Seeks Approval for Commercial Cultivation of GM Banana
- Genome of Australian Round Lime Reveals Key to Citrus Greening Resistance
- Scientists Develop First Gene-Edited Calf Resistant to Bovine Viral Diarrhea Virus
- USDA Helps Bring CRISPR to UH Students to Support Future Food and Agri Workforce
- Synthetic Genetic Manipulation Almost Doubles Lifespan of Cells
- CRISPR Used to Find Antidote for Deadly Mushroom Poison
- Scientists Found a Way to Level Up Nonviral Gene Editing
Read the latest:
- Biotech Updates (September 20, 2023)
- Gene Editing Supplement (September 13, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: