New Method Produces GM Measles Virus Using Only Two Components

A team of researchers from the Paul Erlich Institute in Germany was able to develop a two-component measles virus rescue system that has all the elements ready in the required proportions. Their study is expected to create opportunities for better recombinant measles virus production for research and development.

Genetically modified (GM) measles virus has been used to design viral vector vaccine candidates for protection against other viruses and cancer treatment. But producing it is complex and sometimes inefficient. Using the new method, the German scientists made modified virus genomes on plasmid DNA that multiply autonomously in harmless bacteria. It involves only two components: the plasmid that codes for the modified viral genome and the helper plasmid that bundles all the required helper functions.

The new method works when each component's expression strength adapts to the necessary conditions by coordinated use of the promoters. The sophisticated control is what makes it possible to produce recombinant measles virus based on only two plasmids.

This development makes measles virus research more promising. Further studies were recommended to attain an efficiency of the more complex methods currently being used in virus research.

More information is available in the Journal of General Virology and a news release by Medical Express.