Scientists Characterize Green Tea Genes Encoding Vital EnzymesJanuary 30, 2019
Catechins are major components of the flavonoid pathway in tea in which they are synthesized in four distinct ways under the direct catalysis of two enzymes, leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR).
Vietnamese scientists conducted the cloning and sequence analysis of genes encoding ANR and LAR (namely, CsANR2 and CsLAR1) from the Green Trung Du cultivar. The length of CsANR2 gene is 1,014 bp, encoding 337 amino acids. Comparative analysis of the nucleotide sequences showed that there was limited difference between the CsANR2 gene in the green tea cultivar and the CsANR2 sequence published in Genbank, with nucleotide identity of 98.9–99.6%, and amino acid similarity of 95.7–99.5%. CsANR2 has two major functional regions, the N-terminal glycine-rich domain that functions in association with NAD or NADP (GGTGFVAA); the substrate-specific domain has amino acids involved in enzyme catalysis (S130, Y167 and K171). The results showed that the difference in nucleotide sequences does not lead to amino acid change in the important functional domains of CsANR2.
The length of CsLAR1 gene is 1,029 bp, encoding 342 amino acids. The difference between CsLAR1 of green Trung Du tea and those published in GenBank ranged from 96.3–100% in nucleotide, and 88.3–100% in amino acid sequence. CsLAR1 contains 3 conserved amino acid motifs among species, RFLP, ICCN and THD. However, the ICCN motif of CsLAR1 from green Trung Du tea has a distinct amino acid from the published sequences (I153T). Analysis of the functional domains of CsLAR1 showed that CsLAR1 in tea was conserved in the amino acids linked to the substrate, and the N-terminal glycine-rich domain binding to NADP has a modified amino acid (GACGFIG). More research is needed to study how these amino acid modifications affect CsANR2 and CsLAR1 enzymatic activities.
For more information, read the article in Vietnam Journal of Biotechnology.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Increased Knowledge about GM Foods Leads to More Positive Attitudes, Study Says
- NBMA Solicits Public Comment on the Application for GM Cassava Confined Field Trial
- Nigeria Approves First GM Food Crop for Open Cultivation
- Purdue Research Team Nails Down Important Plant Compound Pathway
- Researchers Discover Molecular Basis of Odor Detection in Plants
- Study Reveals Philippine Lawyers' Perception and Attitude Towards Agri-biotech
- 'Noisy' Gene Atlas to Explain How Plants Survive Environmental Changes
- New Insight into Unique Sugar Transport in Plants
- Scientists Characterize Green Tea Genes Encoding Vital Enzymes
Beyond Crop Biotech
- Use of CRISPR to Control Pest Populations
- Transgenic Pigs Expressing β-xylanase Show Improved Nutrient Utilization
From the BICs
- Vietnamese Farmers Visit Demo Fields of New Maize Varieties
- Good Enough to Eat?: Next Generation GM Crops
- Japanese Scientists Identify Peptide and Receptor Responsible for Plant Root Spacing
- Gene Editing Can be Used to Make Wheat with Safe Gluten
Read the latest:
- Biotech Updates (February 21, 2024)
- Gene Editing Supplement (February 14, 2024)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: