Scientists Develop Modified CRISPR-Cas9 System for Tetraploid PotatoSeptember 19, 2018
Potato's tetraploid genome requires a more powerful genome editing system. Scientist Hiroaki Kusano of Tokyo University of Science and colleagues apply the translational enhancer dMac3 to the Cas9 expression vector to increase the amount of Cas9 proteins produced inside potato cells.
dMac3 is a 161-bp region of the OsMac3 mRNA, which promotes efficiency of translation in the nearby gene. By engineering it to a developed Cas9 system, the researchers find increased efficiency of targeted mutagenesis in the potato gene GBSSI. GBSSI mutants show low amylose starch content in the tubers, a favorable trait in potato. They particularly observe 28% of plants with mutations for all four copies of GBSSI in the tetraploid genome, emphasizing the usefulness of the enhancer in polyploid genome editing. The researchers also elaborate on the enhancer's contribution to more efficient targeted mutagenesis in the paper.
For more information, read the article in Nature Scientific Reports.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- UN: Global Hunger Continues to Rise
- Kenyan Scientists Concerned Perceptions on GMOs May Slow Down GM Crops Commercialization
- Researchers to Improve Iron Toxicity Tolerance and Yield of Rice in Africa
- Burkina Faso Farmers Call for Return of Bt Cotton
- Scientists Crack A Code that Could Help Nourish the World
- How Plants Signal Danger Long Distances
- International Team Delivers Intermediate Wheatgrass Reference Genome
- Australian OGTR Invites Comments on GM Canola Field Trial
- John Innes Centre Leads Group's Call for Clarity after EU Ruling on Gene-Edited Crops
- Vietnamese Scientists Develop Carotenoid-rich Maize
- Candidate Genes Related to Drought Stress Identified in Cotton
Plant Breeding Innovations
- CRISPR-Cas9 Reveals New Findings on miRNAs in Rice
- Bacterial Speck Resistance Developed in Tomato Using CRISPR-Cas9
- Rice Plant Architecture Engineered Using CRISPR-Cas9
- Scientists Develop Modified CRISPR-Cas9 System for Tetraploid Potato
Beyond Crop Biotech
- Researchers Use CRISPR Technology to Treat Beta Thalassemia Patients
- Thousands of Breast Cancer Gene Versions Classified Using CRISPR-Cas9
- Scientists Engineer Powerful Weapon Against Antibiotic-Resistant Bacteria
From the BICs
- ISAAA Report on Biotech Crop Adoption Presented to Multiple Stakeholders in Japan
Subscribe to CBU: