Scientists Edit Gene for Plant Height in Tomato
September 5, 2018 |
Researcher Laurence Tomlinson and colleagues from Norwich Research Park in UK and University of Minnesota in the USA used CRISPR-Cas9 to edit the gene coding for DELLA proteins in tomato. Tomatoes are economically important and widely studied for traits such as disease resistance, fruit shape, and color. Aiming for a tomato with a dwarf trait will reduce the need for additional mechanical support, pruning, and side support management.
Results of the study showed the generation of three transgene-free heritable genotypes through genome editing. The first genotype is homozygous PROD/PROD, which confer the dwarf trait and partial responsiveness to GA; the second one is heterozygous PROD/PRO, which had intermediate height when young but dwarf when matured. The mutation introduced is therefore dominant. The third genotype is also a loss-of-function mutation that conferred traits like the DELLA-silenced plant.
For more information, read the article in Plant Biotechnology Journal.
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