CRISPR-Cas9 Found Applicable to AlfalfaMarch 14, 2018
RNA-guided genome engineering using CRISPR-Cas9 enables a variety of applications in plants. Successful application and validation of the CRISPR technique in a multiplex genome, such as that of alfalfa will lead to major advances in the improvement of this crop.
The team of Ruimin Gao from Agriculture and Agri-Food Canada used the CRISPR-Cas9 system to target the squamosa promoter binding protein like 9 (SPL9) gene in alfalfa. Due to the complexity of the alfalfa genome, the team first screened large populations of CRISPR-modified plants to select plants with high genome editing rates. These plants were then subjected to further analyses and mutations on the desired target site were verified in these selected plants.
The team successfully applied the CRISPR-Cas9 technique to edit the SPL9 gene in a multiplex alfalfa genome. However, the overall efficiency of CRISPR in the polyploid alfalfa genome was lower compared to other plant genomes. Further refinement of the CRISPR technology system will be required for more efficient genome editing in alfalfa.
For more information on this study, read the article in Planta.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- African Seed Companies Encouraged to Invest in Biotechnology
- Ugandan Journalists Vow to Change the Rhetoric against Science
- Scientists Engineer Crops to Use 25% Less Water; Resist Drought
- Researchers Discover How Weeds Develop Glyphosate Resistance
- Forty Years of Data Quantifies Benefits of Bt Corn Adoption Across Different Crops
- Report Confirms Ban on GM Crops in South Australia Does Not Deliver Any Benefits to Farmers
- Japan to Finalize GE Labeling Requirements Soon
- Study Quantifies Cost of Delayed GM Canola Adoption in Australia
- GMOinfo.eu Website is Launched
- Researchers Develop GM Sugarcane with Pyramided Insecticidal Proteins
- Maize Gene Improves Drought Tolerance and Grain Yield in Rice
- RNAi Confers Resistance Against Sugarcane Mosaic Virus
Plant Breeding Innovations
- CRISPR-Cas9 Found Applicable to Alfalfa
- Scientists Study Seed Germination in Lettuce Using CRISPR
- Chinese Researchers Study Abiotic Stress Tolerance Genes in Rice
- Safer Potatoes Developed Using CRISPR-Cas9
Beyond Crop Biotech
- First-Ever Transgenic Tick to Help Fight Tick-Borne Diseases
- 2nd Biotechnology World Symposium
Subscribe to CBU: