Production of Isopropanol–Butanol–Ethanol Mixture by Engineered Clostridium acetobutylicumJanuary 31, 2018
The formation of by-products, mainly acetone, in the acetone–butanol–ethanol (ABE) fermentation significantly affects the yield. The team led by Chao Wang of Nanjing Tech University in China genetically-engineered Clostridium acetobutylicum to eliminate acetone production. The team also altered ABE fermentation to isopropanol–butanol–ethanol (IBE).
After introduction of secondary alcohol dehydrogenase into C. acetobutylicum XY16, the engineered XY16 completely eliminated acetone and converted it to isopropanol, indicating great potential for the production of IBE mixtures. Under the optimal pH level of 4.8, the total IBE production was significantly increased from 3.88 to 16.09 g/L, with final yields of 9.97, 4.98 and 1.14 g/L for butanol, isopropanol, and ethanol, respectively.
Furthermore, calcium carbonate could be both a buffer and activator for NAD kinase (NADK). Supplementation of calcium carbonate further improved IBE production to 17.77 g/L with 10.51, 6.02, and 1.24 g/L of butanol, isopropanol, and ethanol, respectively.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Hunger in Conflict Areas Continues to Escalate
- Namibia Launches First GM Detection Lab
- African Women Vow to Rally Behind Biotech
- Scientists Explain How A Protein Helps Plants "Muscle Up" Bacteria in the Cold
- Arctic® Fuji Apple Gets Canadian Approval
- Importance of IP and Its Application to Biotech Emphasized
- Gene Editing and Genomics Discussed at UP Los Baños Seminar
- "Wild" Genes Open Up Opportunities for Better Rice Varieties
- UZH Scientists Develop Wheat Lines Resistant to Powdery Mildew
- Researchers Identify Plum Pox Virus Susceptibility Genes in Apricot
- Walnut Gene Involved in Cadmium Stress Response
Beyond Crop Biotech
- Non-coding DNA Vital for Brain Development
- Australian OGTR Invites Comments for Field Trial of GM Buffalo Grass
- 10th World Potato Congress
- Researchers Perform Allele Exchange at EPSPS Locus in Cassava using CRISPR-Cas9
Read the latest:
- Biotech Updates (March 22, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: