Heat Stress Increases the Efficiency of CRISPR-Cas9 Mutagenesis in PlantsNovember 29, 2017
The CRISPR-Cas9 system has allowed the possibility to engineer targeted mutations in eukaryotic genomes. However, the efficiency of mutagenesis and the generation of off-target mutations vary greatly between different organisms. Chantal LeBlanc of Yale University now reports that plants subjected to heat stress at 37°C show higher frequencies of CRISPR-induced mutations compared to plants grown at the standard temperature of 22°C.
LeBlanc's team found that targeted mutagenesis by CRISPR-Cas9 in Arabidopsis has increased by approximately five times in somatic tissues and up to 100 times in germlines upon heat treatment. This effect of temperature on the mutation rate was also found to be not limited to Arabidopsis, as a similar increase was observed in CRISPR-edited Citrus plants exposed to heat stress at 37°C.
Analysis revealed that the Cas9 protein from Streptococcus pyogenes (SpCas9) was more active in creating double-stranded DNA breaks at 37°C than at 22°C, indicating a potential contributing mechanism for the effect of temperature on CRISPR-Cas9.
This study reveals the importance of temperature in improving SpCas9 activity in eukaryotes, and provides a simple method to increase on-target mutagenesis in plants using CRISPR-Cas9.
For more information, read the article in The Plant Journal.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- AUC and FAO Ask African Gov'ts to Promote Agri-Biotech to Help Combat Food Insecurity
- Corn Research Exposes Mechanism Behind Gene Silencing
- Argentina Approves New Biotech Soybean
- EU Ban on GM Crops Hurting Productivity, says Retired Professor
- EFSA Releases Scientific Opinion on Four-Stack GM Maize
- Biotech Cotton Co-expressing Vip3AcAa and Cry1Ac Confers Protection against Cry1Ac-resistant Bollworm
- Cgl2 Gene Involved in Cuticular Wax Synthesis in Cabbage
- SlJAZ2 Overexpression Accelerates Reproductive Growth in Tomato
Beyond Crop Biotech
- DARPA Explores on Using Plants to Detect Security Threats
- Researchers Pinpoint the Regulator of Phenylalanine Synthesis in Maritime Pine
- Scientists Study Genes Involved in Triterpenoid Synthesis in Birch
- 3rd Biennial National Agricultural Sciences Conference and Exhibition
- Biotech Country Facts and Trends
- Heat Stress Increases the Efficiency of CRISPR-Cas9 Mutagenesis in Plants
- Study Shows CRISPR Mainly Used to Develop High-yielding, Healthier, and Stress-resistant Crops
Read the latest:
- Biotech Updates (May 24, 2023)
- Gene Editing Supplement (May 24, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: