Overexpression of a Blueberry DDF1 Gene Confers Increased Tolerance to FreezingJune 21, 2017
The CBF/DREB 1 increases freezing tolerance in woody plants but is associated with dwarfed plants and delayed flowering. These same changes were observed when Arabidopsis DWARF AND DELAYED FLOWERING 1 (DDF1) was overexpressed. However, DDF1 orthologs have not been studied in woody plants.
Michigan State University's Guo-qing Song investigated the overexpression of blueberry (Vaccinium corymbosum) DDF1, named VcDDF1. The VcDDF1 overexpression resulted in enhanced freezing tolerance in tetraploid blueberry plants and did not result in significant changes in plant size and flowering time. Analysis revealed differentially expressed (DE) genes in the pathways of cold-response, plant flowering, DELLA proteins, and plant phytohormones between the transgenic and non-transgenic blueberry.
The increase in freezing tolerance was associated to the expression of cold-regulated genes and the ethylene pathway genes. The unchanged plant size, dormancy and flowering were due to the minimal effect of the VcDDF1-OX on the expression of DELLA proteins, flowering pathway genes, and the other phytohormone genes. The differentially expressed genes in auxin and cytokinin pathways also suggest altered plant tolerance to drought and high salinity.
The overexpression of VcDDF1 or its orthologues can be a new approach to increase freezing tolerance of woody plant species.
For more on this study, read the article in BMC Plant Biology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- GM Crop Approvals Boost Bioengineered Foods Market, Report
- African Countries Launch the Global Report on Commercialized Biotech/GM Crops
- Student to Boost Vitamin A Content of Chickpea
- Research Found Way to Improve Nitrogen Fixation in Legumes
- Farmer Perspective Brought to Biotech Regulatory Discussions
- American Soybean Association: Biotech Essential to Feed 9.7B People in 2050
- China Approves Import of GM Crops from the U.S.
- Heat Tolerant Lentils Endure Increasing Global Temperatures
- Chinese Academy of Sciences Partners with Bayer to Improve Wheat Yields
- Researchers Unlock the Genetic Secrets of Plant Cell Walls
- Researchers Study Cotton Kinesin Gene GhKIS13A1
- Overexpression of a Blueberry DDF1 Gene Confers Increased Tolerance to Freezing
Plant Breeding Innovations
- Multi-Site Genome Editing in Cotton using CRISPR-Cas9 System
Beyond Crop Biotech
- Kenya's Imaginature Seeks Approval for Field Trial of GE Baby's Breath Flowers
- Defense Secret of E. coli Revealed
Read the latest:
- Crop Biotech Update (December 1, 2022)
- Genome Editing Supplement (November 23, 2022)
- Gene Drive Supplement (December 1, 2022)
Subscribe to CBU: