CRISPR-mediated Targeted Mutagenesis in Grape
May 31, 2017| |
RNA-guided genome editing using the CRISPR-Cas9 system has been applied successfully in several plant species. However, there have been only a few reports on the use of any of the current genome editing approaches in grapes (Vitis vinifera L.).
The team of Ikuko Nakajima from the National Agriculture and Food Research Organization in Japan successfully performed targeted mutagenesis in grape using the CRISPR-Cas9 system. The team designed a construct targeting the phytoene desaturase (VvPDS) gene in grapes and transformed it into embryonic calli.
The resulting regenerated plants exhibited albino leaves. DNA sequencing confirmed that the VvPDS gene was mutated at the target site in regenerated grape plants. Interestingly, the mutated cells were higher in lower, older, leaves compared to that in newly appearing upper leaves.
For more on this study, read the article in PLOS One.
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