Knockdown of Mythimna separata Chitinase Genes via Oral delivery of RNAi effectorsFebruary 15, 2017
RNAi (RNA interference) technology has been used to develop insect and disease resistant crops. Mythimna separata belongs to noctuidae family of lepidoptera and is posing threat to crops of economic importance. Recently, outbreaks of M. separata severely threaten corn production in Northern China, calling for new control approaches. The team of Oyunchuluun Ganbaatar from Inner Mongolia University chose to target chitinase genes as they were expressed predominantly in the gut tissue and were reported to be ideal silencing targets in several insect species.
Interfering sequences against the target genes were cloned into the L4440 vector to produce sequence specific dsRNAs (double-stranded RNAs). These were then transformed into Escherichia coli strain HT115 (DE3). The bacteria were mixed with artificial diet and were fed to M. separata.
Analysis showed that expression level of target MseChi1 and MseChi2 genes in gut tissue of M. separata were downregulated after oral delivery of engineered bacteria expressing the corresponding dsRNA. Furthermore, knockdown of MseChi1 and MseChi2 resulted in increased mortality and reduced body weight of the feeding larvae.
This study reports a simple and low cost experimental procedure to silence M. separata endogenous gene expression. This provides both an experimental foundation for using RNAi technology to control M. separata and also a tool for loss-of-function studies of genes in this species.
For more on this study, read the article in BMC Biotechnology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Church Leaders in Nigeria Support Agri-Biotech Research
- Researchers Discover New Link in Fight Against Billion-Dollar Threat to Soybean
- Mutant Maize Has Key Information to Understanding Plant Growth
- Scientists Explain How Plants Resist Drought
- Researchers Sequence Quinoa Genome
- Scientists to Test GE Bananas Resistant to TR4 in the Northern Territory, Australia
- Australian OGTR Issues License for Field Trial of GM Indian Mustard
- Research Explains Plant Tissues' Sense of Direction
- Scientists Develop Rubber-Enriched Dandelion through Metabolic Engineering
Plant Breeding Innovations
- Researchers Review Targeted Genome Editing Techniques in Horticultural Crops
Beyond Crop Biotech
- First Gene Drive in Mammals Could Help Eradicate Invasive Rodents
- Knockdown of Mythimna separata Chitinase Genes via Oral delivery of RNAi effectors
- BIO International Convention
- Plant Transformation & Biotechnology IV
- Genome Editing and the Future of Farming
Subscribe to CBU: