MdMLO19 Gene Knockdown in Apple Reduces Susceptibility to Powdery Mildew
May 18, 2016| |
Varieties resistant to powdery mildew (PM) caused by Podosphaera leucotricha are a major component of sustainable apple (Malus domestica) production. Resistance can be achieved by knocking-out susceptibility S-genes, which can be singled out from members of the MLO (Mildew Locus O) gene family. Candidate genes include the MLO S-genes MdMLO11, MdMLO18 and MdMLO19, which were found upregulated upon PM inoculation.
Scientists from the Fondazione Edmund Mach in Italy and Wageningen University and Research Centre in the Netherlands report on the RNA interference knockdown of MdMLO11 and 19, as well as the complementation of resistance with MdMLO18 in the Arabidopsis thaliana triple mlo mutant.
The knockdown of MdMLO19 reduced PM severity by 75%, while the knockdown of MdMLO11, alone or in combination with MdMLO19, did not result in any reduction or additional reduction of susceptibility. The test in Arabidopsis excluded a role for MdMLO18 in PM susceptibility.
MdMLO19 plays an important role in apple PM susceptibility and its knockdown induces a very significant level of resistance.
For more information, read the full article in Plant Biotechnology Journal.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- U.S. National Academies Find Biotech Crops Not Harmful to Human Health and the Environment
- Glyphosate Unlikely Carcinogenic to Humans, Says Joint WHO/FAO Committee Report
- Cairo University Celebrates Biotech Day 2016
- FSU-Cornell Research Team Discovers 'Dark Matter' in Maize Genome
- Newly Discovered Stem Cell Pathway Will Increase Yields of Maize and Staple Crops
- Parliamentary Report Confirms Importance of Agricultural Innovation in Australia
- Bt Cotton Adoption Creates More Employment Opportunities in Female Laborers in Pakistan
- Chinese Scientists Evaluate Performance of Plant Gene as a Selectable Marker for Rice Transformation
- Agri Minister Matia Chowdhury Speaks on Biotech Efforts in Bangladesh
- First State of the World's Plants Report Launched
-
Research Highlights
- Asian Corn Borer Parasite Not Sensitive to Bt Toxin
- MdMLO19 Gene Knockdown in Apple Reduces Susceptibility to Powdery Mildew
- Researchers Analyze Rice QTL for Grain Size and Yield
-
Beyond Crop Biotech
- Scientists Genetically Engineer First Zika Virus Clone
-
Announcements
- SolGenomics Conference
-
Read the latest: - Biotech Updates (November 27, 2024)
- Gene Editing Supplement (November 27, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet