E. coli-based Production of Recombinant Ovine Angiotensinogen
April 27, 2016| |
Angiotensinogen (ANG) is a precursor of angiotensin,which regulates blood pressure and electrolyte balance. Ovine ANG (oANG) from sheep plasma has been shown to be a better substrate for human renin. To expand the availability of oANG, a team of researchers from Gifu university in Japan, aimed to produce recombinant oANG using Escherichia coli.
By expressing oANG at 37°C from a tac promoter, the team significantly elevated the ratio of soluble to insoluble recombinant oANG. The team then purified tac-expressed recombinant oANG to homogeneity. The E. coli-expressed recombinant oANG was found similar to that of oANG expressed in CHO cells. Analysis also revealed that recombinant oANG expressed in E. coli functions the same as a human renin substrate.
For more information, readthe full article on BMC Biotechnology.
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