Isolation of Anthocyanin Pathway Regulator from Horny Goat Weed
March 30, 2016| |
Anthocyanin biosynthesis is regulated by a series of transcription factors. Previous studies have discovered a MYB transcription factor involved in regulation of the anthocyanin pathway from the traditional Chinese medicinal herb, horny goat weed (Epimedium sagittatum). However, it was expressed mostly in leaves.
Jingxin Chen, together with Zheijang University researchers, isolated another MYB transcription factor, EsAN2, from flowers of E. sagittatum. EsAN2 was found to be expressed in both flowers and flower buds. Ectopic expression of EsAN2 in tobacco significantly enhanced anthocyanin biosynthesis and accumulation both in leaves and flowers. Furthermore, most structural genes of the anthocyanin pathway were strongly upregulated in old leaves of tobacco overexpressing EsAN2 compared to the control plants.
These results suggest that EsAN2 is involved in regulation of the anthocyanin biosynthesis in Epimedium flowers. Identification and characterization of EsAN2 provide insight into the coloration of Epimedium flowers and a potential candidate gene for engineering of flavonoids.
For more information, read the article on Plant Cell Reports.
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