Overexpression of Amur Grape Gene Confers Abiotic Stress Tolerance to Arabidopsis
January 6, 2016| |
The plant-specific GRAS transcription factor family regulates diverse processes involved in plant growth, development, and stress responses. The team of Yangyang Yuan from Chinese Academy of Sciences isolated and characterized, VaPAT1, a GRAS gene from Amur grape (Vitis amurensis).
VaPAT1 was found similar to function in stress resistance. Its transcription was induced by stress-related phytohormone abscisic acid (ABA) and various abiotic stress treatments such as cold, drought, and high salinity. However, it was repressed by exogenous gibberellic acid (GA) application.
Overexpression of VaPAT1 increased abiotic stress tolerance in transgenic Arabidopsis. The VaPAT1-overexpressing lines also accumulated higher levels of proline and soluble sugar under stress conditions. Stress-related genes also showed higher expression in VaPAT1 transgenic lines than in wild types under normal growth conditions.
These indicate that VaPAT1 is a positive transcriptional regulator involved in grapevine abiotic stress responses.
For more information on the study, read the article in Plant Cell Reports.
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