Expression of a Recombinant Microbial Transglutaminase in Escherichia coli
September 23, 2015| |
Bacterial transglutaminases are required as industrial reagents for the modification of proteins in different industries. Barbara Salis of the Sardinia Scientific and Technological Park led researchers in studying the production in Escherichia coli of a recombinant transglutaminase from Streptomyces mobaraensis (MTGase).
Thermoinducible and constitutive expression vectors expressing Met-MTGase were constructed for the transformation of E. coli strains. After transformation, competent Escherichia coli K12 strains were evaluated to select the best conditions for Met-MTGase expression. The two best performing fusion protein systems were from E. coli strains NP668/1 and NP650/1. The fusion proteins from both strains were then compared the protein product of wild types.
The fusion protein from NP668/1 strain, which was found to perform comparable to the wild-type enzyme, was then selected as a candidate for producing microbial transglutaminase for industrial applications.
For more on the study, feel free to visit the full article on BMC Plant Biotechnology.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- 'Tree of Life' for 2.3 Million Species Released
- USDA ARS Honors Four Experts as Science Hall of Fame
- GE Crops are Vital to Meet Food Demand, South Asia Experts Say
- Wheat without GM will Lead to Food Shortages, says World Food Prize Winner
- Australia Court Rules in Favor of GE Crop Farmers
- Scientists Clone Rare Gene to Enhance Rice Grain Yield
- Photosynthesis Gene Could Help Plants Stay Healthy in Stressful Conditions
-
Research Highlights
- Identification of Powdery Mildew Susceptibility Genes in Cultivated Solanaceous Crops
- DWARF Overexpression Alters Phytohormone Levels, Plant Architecture, and Carotenoid Accumulation in Tomato
-
Beyond Crop Biotech
- Research Team Identifies 14 Genomes of Colombian Cocoa
- Braconid Wasps as Accidental Genetic Engineers
- Expression of a Recombinant Microbial Transglutaminase in Escherichia coli
-
From the BICs
- UBIC Supports the 2015 National Science Olympiad
-
Resources
- Biotech Trait Annual Updates 2014 and Updated Pocket Ks Now Available
- Infographic: International Science Organizations on Crop Biotech
-
Read the latest: - Biotech Updates (April 30, 2025)
- Gene Editing Supplement (April 30, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet