Development of Cellobiose-Degrading Yarrowia lipolytica Strain through Overexpression of Endogenous Genes
August 5, 2015http://www.biotechnologyforbiofuels.com/content/8/1/109
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Yarrowia lipolytica is unable to grow on cellobiose. Hence, engineering cellobiose-degrading ability into this yeast is a vital step towards the development of cellulolytic catalysts suitable for bioprocessing. Researchers from the University of Toulouse, INRA, and AgroParisTech in France identified six genes encoding β-glucosidases in the Y. lipolytica genome.
Each of the six genes was expressed in Y. lipolytica JMY1212 Zeta. However, only the strains overexpressing BGL1 and BGL2 were able to degrade cellobiose. The two β-glucosidases were then purified and characterized.
Bgl1 displayed a higher catalytic efficiency on cellobiose than Bgl2. Significantly, a Y. lipolytica strain co-expressing both BGL1 and BGL2 grew better than strains expressing only a single BGL. The growth rate and biomass yield of the strain co-expressing BGL1 and BGL2 were also found similar to that of the control grown on glucose.
The resulting Y. lipolytica developed in the study will be vital towards the creation of a cellulolytic yeast strain to be used for lipid production from lignocellulosic biomass.
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