Development of HSSL-Tolerant Scheffersomyces stipitis Through Evolutionary Engineering
April 1, 2015http://www.biotechnologyforbiofuels.com/content/8/1/50/abstract#
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Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping that is rich in xylose, which can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL contains acetic acid and lignosulfonates that inhibit yeast growth. Ana Xavier from Universidade de Aveiro in Portugal aimed to use evolutionary engineering to obtain variants of S. stipitis with increased tolerance to HSSL.
A continuous reactor with gradually increasing HSSL concentrations was used for 382 generations. From the final population, a stable clone (C4) was isolated and characterized in 60% HSSL. The C4 isolate was then compared with both the parental strain and the final population. The final population and C4 were able to grow in 60% HSSL. The C4 also exhibited higher substrate uptake rates, higher ethanol efficiency and improved ethanol yield.
S. stipitis was successfully adapted to 60% HSSL and a stable isolate with improved activity in HSSL was also obtained from the final population. C4 will be crucial for the production of bioethanol using HSSL.
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