Medicinal Plant Swertia Goes In vitro
April 23, 2014| |
Swertia chirayita, a medicinal plant in India, is economically important due to its bitterness, antihelminthic, hypoglycemic, hepatoprotective, and antiviral properties. There have been few studies about tissue culture of S. chirayita and still no studies regarding in vitro flowering. An in vitro flowering system, if developed in S. chirayita, may be able to overcome problems associated with in vitro flower growth and development as well as open the idea for in vitro fruit and seed production.
Axillary bud explants were used to study in vitro flowering and effective micropropagation protocols for Swertia chirayita. The Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) 1.0 and adenine sulfate 70.0 was found optimum for production of multiple shoots. Incubation of flowering cultures on BAP-supplemented medium was found necessary for flowering. In vitro flowering was found to be influenced by subculture duration, photoperiod and carbon source. Mature flowers were observed when the cultures were maintained in the same medium.
In vitro flowering could potentially become an important tool in tissue culture due to its ability to shorten the life cycles of plants. This technique can also serve in mass productions of specific organs with unique compounds for pharmaceutical and other uses.
Read more at http://www.hindawi.com/journals/btri/2014/264690/.
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