Comparison of Agrobacterium tumefaciens Strains for the Genetic Transformation of Tomato
November 7, 2012| |
Different Agrobacterium tumefaciens strains have been used in tomato transformation, which had varying efficiencies. To find out which of the commonly used strain is the most efficient, V.J. Chetty from University of California Riverside and other scientists conducted a study. They tested the ability of strains GV3101, EHA105, AGL1, and MP90 to transform Micro-Tom and incorporate specific copy number in host plant cells. Results showed Agrobacterium strain GV3101 showed the highest transformation rates and lowest percentage of plants with single transgene insertions. On the other hand, strain MP90 had the least transformation capacity but the highest frequency of plants with single transgene copies. Strain EHA105 exhibited the best combination of high transformation efficiency and single insertional events of transgenes, which is favorable for functional genomics and biotechnological applications in tomato.
Read more about the study at http://link.springer.com/article/10.1007%2Fs00299-012-1358-1?LI=true.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- IFAD's New Program Aims at Smallholder Farmers' Adaptation to Climate Change
- UK, IRRI, BMGF Allot $14M for Further Research on C4 Rice
- Zimbabwe to Start Growing Drought-tolerant Maize Soon
- African Stakeholders Tour Bt Cotton Fields in Burkina Faso
- Study Details Essential Role of Trust in Agricultural Biotech Partnerships
- Scientists Develop Witchweed Resistant Maize Variety
- OFAB Africa Launches Burkina Faso Chapter
- Disease-causing Mechanism of Cochliobolus victoriae Fungus in Plants
- Scientists Find Aphid Resistance in Black Raspberry
- Friendly Fungus Tapped to Induce Pest Resistance in Beans, Cassava
- Mitsubishi Funds Bioenergy Research in Sarawak Algae
- Press Freedom and Biotech Policy on News Coverage of GM Crops
- Vietnam to Grow More Corn Including GM
- New Rice Varieties to Increase Upland Farmers' Yield in the Philippines
- Scientists Reveal Process in Plants-Soil Microbes Interaction
- Parent Plants Provide Antibodies to Offspring, Research Finds
- Biologists Discover Process Affecting Fruit Ripening in Crops
- Plants Recognize Pathogenic and Beneficial Microorganisms
-
Research Highlights
- Comparison of Agrobacterium tumefaciens Strains for the Genetic Transformation of Tomato
- Increasing Lactostatin in Biotech Rice Using Soybean Protein as Carrier
-
Beyond Crop Biotech
- First Yeast Gene Discovered to Improve Beer Froth
-
Announcements
- Basic Wheat Training
- BioVeg2013
-
Read the latest: - Biotech Updates (September 4, 2024)
- Gene Editing Supplement (August 28, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet