Species: Blunt snout sea bream (Megalobrama amblycephala)

Country of 1st Author: China
Year: 2020

Institute	Shanghai Ocean University Shanghai 201306 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The fertilized eggs (1–2 cell stage) were injected with 1 nL mixture of gRNA (100 pg) and Cas9 protein (900 pg).
Remarks	mstna and mstnb
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	three target sites (targets 1, 2, and 3) to generate knock out mutants within the mature mRNA sequence of mstna or mstnb that were located in exon 1, exon 3, and exon 3, respectively.

References, Documents, and Links

• Disruption of mstna and mstnb gene through CRISPR/Cas9 leads to elevated muscle mass in blunt snout bream (Megalobrama amblycephala)