Species: Channel catfish (Ictalurus punctatus)

Country of 1st Author: United States
Year: 2016

Institute	Auburn University Auburn, AL 36849 US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Three sets of zinc finger nuclease (ZFN) plasmids targeting the luteinizing hormone (LH) gene were designed and electroporated into one-cell embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
LH luteinizing hormone	This gene encodes the receptor for both luteinizing hormone and choriogonadotropin.	Reproduction	ZFN	The ZFN plasmids of each set were prepared separately for the purpose of double electroporation (Su 2012; Dunham and Winn 2014). Equal amounts of both left and right ZFNs were mixed together and diluted with 2 ml saline (0.9 % NaCl) to the final concentrations of 10, 25, and 50 µg/ml for the first electroporation of sperm

References, Documents, and Links

• Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation