Species: Chicken (Gallus gallus)

Country of 1st Author: Czech Republic
Year: 2021

Institute	Czech Academy of Sciences Videnska 1083, 142 20 Prague CZ
Editing Method	Other
SDN Type	SDN-1
Delivery	PGCs (2.5 × 105) were suspended in Nucleofector Solution V (Lonza) and mixed with 10 µg of pX458-TVA in total volume of 100 µL. Nucleofection was performed with the AMAXA nucleofector
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Tva	Resistance to Avian Leukosis Virus Subgroups A and K	Biotic Stress	CRISPR Cas9 (spCas9)	gene editing in chicken primordial germ cells and orthotopic transplantation of edited cells into the testes of sterilized recipient roosters

References, Documents, and Links

• Knock-Out of Retrovirus Receptor Gene Tva in the Chicken Confers Resistance to Avian Leukosis Virus Subgroups A and K and Affects Cobalamin (Vitamin B12)-Dependent Level of Methylmalonic Acid