Species: Chicken (Gallus gallus)

Country of 1st Author: Germany
Year: 2020

Institute	Technical University Munich Freising DE
Editing Method	Other
SDN Type	SDN-2
Delivery	PGCs were co-transfected with a CRISPR/Cas9 vector expressing a single guide RNA and Cas9-2A-eGFP in combination with a single?stranded oligodeoxynucleotide (ssODN). The ssODN served as DNA repair template to specifically target the W38 coding region of chNHE1 within Exon1
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
chNHE1 chicken Na+/H+ exchanger type 1		Biotic Stress	CRISPR Cas9 (spCas9)	precise deletion of chNHE1 W38

References, Documents, and Links

• Acquiring Resistance Against a Retroviral Infection via CRISPR/Cas9 Targeted Genome Editing in a Commercial Chicken Line