Species: Chicken (Gallus gallus)

Country of 1st Author: South Korea
Year: 2023

Institute	Depatment of Bio Life Science, ShinGu University Seongnam 13174 KR
Editing Method	Other
SDN Type	SDN-3
Delivery	For the Z chromosome KI experiment, 7.5 µL Lipofectamine 3000 Reagent were diluted in 250 µL OPTI-MEM (Invitrogen, USA), and the Cas9 expression plasmid, Z chr gRNA, and donor GFP KI vector (1.5:1:3.0 µg) were mixed with Lipofectamine P3000 Reagent in 250 µL OPTI-MEM at room temperature
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
GFP Green Fluorescent Protein	a marker of gene expression and protein targeting in intact cells and organisms	Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Production of chickens with green fluorescent protein-knockin in the Z chromosome and detection of green fluorescent protein-positive chicks in the embryonic stage