Species: Goat (Capra hircus)

Country of 1st Author: United States
Year: 2020

Breed Strain	Nubian × Boer
Institute	Washington State University Pullman, WA 99164 US
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	To generate NANOS2 KO males for phenotyping and SSCT, somatic cell nuclear transfer (SCNT) was used (19) with the nuclear DNA donor deriving from XY fetal fibroblasts of Nubian × Boer crossbred male goats that were modified by CRISPRCas9 editing to possess 16-bp and 23-bp NANOS2 ? alleles (SI Appendix,Fig. S6). In total, 125 one-cell-stage cloned embryos were generated and surgically transferred into seven estrous synchronized recipients.At40to45dof gestation, five recipients were confirmed preg
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
NANOS2 Nanos C2HC-Type Zinc Finger 2	Germline ablated males to act as surrogate sires	Yield	CRISPR Cas9 (spCas9)	For pigs 150-bp and 4-bp ? NANOS2 alleles and for goats 16 bp and 23 bp NANOS alleles. For cattle 11 or 300 bp

References, Documents, and Links

• Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2 knockout males